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让 exosome浓缩,分析不再是难题
发布时间:2019-08-22 21:56 点击次数:
Exosomes 为细胞经外吐作用 (exocytosis pathway) 所产生的小囊泡 (membrane vesicles),颗粒直径为:40–100 nm;在 血液、尿液、唾液、羊水、腹水等动物体液,均有它的踪迹。Exosomes 表面带有特殊的蛋白质标记 ( 如 CD63、CD9、 CD8及TSG101),主要由造血细胞 (hematopoetic cells)、淋巴球细胞 (B & T lymphocytes) 及巨噬细胞 (marcrophages) 分泌至体液里;并有研究指出外皮层細胞 (epithelial cells) 及肿瘤细胞 (tumor cells) 也会分泌大量 exosomes。
Exosomes 除了负责蛋白质运输 (protein trafficking),在 2007 年文献更指出可以运送 mRNA 及 microRNA,保护两者不 被 RNase 分解,进而影响邻近细胞的蛋白质表現。由于 microRNA 在 exosomes 中大量存在,肿瘤细胞也常大量分泌 exosomes,因此,microRNA 在 cancer therapy 逐渐成为新的癌化指标或治疗方向;也因此,如何纯化 exosomes 更显得重要。
浓缩,分析步骤:
步骤一,ExoQuick Exosome Precipitation Solution-Exosomes浓缩,
一 外泌体的分离
SBI公司研究和开发了一款基于多聚体形式的Exosome提取试剂,能够快速有效地提取及分离血清和肿瘤组织样本中的Exosome囊泡。这些多聚体能够形成类似于网状结构,从而将60-180nm直径的微泡分离出来。其纯度和产量皆优于传统方法。
(一)外泌体分离纯化产品选择
分离完整 Exosome | ExoQuick: | 适合从富含Exosome样本中提取exosome如:血清、腹水 |
ExoQuick-TC: | 适合从含量较少的样本中提取exosome如:细胞培养上清、尿液、脑脊液 | |
ExoQuick Plasma Kit: | 从血浆中提取exosome(试剂盒中含Thrombin及ExoQuick试剂) | |
ExoQuick-CG: | 药品生产管理规范标准级别的用于临床前的应用研究(适用于各种培养液、体液如尿液、唾液、脑脊液等等样本的Exosome提取) | |
ExoQuick-LP | 预处理血清或血浆样本,以出去脂蛋白 |
货号 | 产品 | 规格 | 价格(元) |
EXOQ5A-1 | ExoQuickExosome precipitation solution | 5ml(75T) | 4546.00 |
EXOQ20A-1 | ExoQuickExosome precipitation solution | 20ml(300T) | 14662.00 |
EXOTC10A-1 | ExoQuick TC Exosome precipitation solution | 10ml | 4529.00 |
EXOTC50A-1 | ExoQuick TC Exosome precipitation solution | 50ml | 15214.00 |
EXOCG50A-1 | ExoQuick-CG™ Exosome Precipitation Solution | 50ml(30R) | |
EXOQ5TM-1 | ExoQuick Plasma prep and Exosome Precipitation Kit | 75T | 10341.00 |
关联产品
货号 | 产品 | 规格 | 价格(元) |
TMEXO-1 | Thrombin Plasma prep for Exosome Precipitation | 100T | 6930.00 |
EXOLP5A-1 | ExoQuick-LP Lipoprotein PreClear&Exosome Precipitation Kit | 10T | 6279.00 |
EXO-FBS-50A-1 | Exosome-depleted FBS Media Supplement(无外泌体血清) | 50ml | 2546.00 |
EXO-FBS-250A-1 | Exosome-depleted FBS Media Supplement(无外泌体血清) | 250ml | 11000.00 |
SBI为您推荐Exo-Flow抗体磁珠试剂盒,该试剂盒能够捕获Exosome特定的表面标记蛋白,利用流式分选技术,从而分离出不同亚型的Exosome。试剂盒中提供的9.1μM直径的Exo-Flow磁珠与传统的4μM Dynabeads磁珠相比,能够更好地捕获Exosome,特别适用于数量较少的Exosome样本,并且有多种标记抗体供选择。
产品信息
货号 | 产品 | 规格 | 价格(元) |
EXOFLOW100A-1 | CD9 Exo-Flow capture kit (Magnetic streptavidin beads, CD9-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW150A-1 | TetraspaninExo-Flow combo capture kit (Magnetic streptavidin beads, CD9, CD63, and CD81-biotin capture antibodies, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 9995.00 |
EXOFLOW200A-1 | CD31 Exo-Flow capture kit (Magnetic streptavidin beads, CD31-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW210A-1 | CD44 Exo-Flow capture kit (Magnetic streptavidin beads, CD44-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
XOFLOW300A-1 | CD63 Exo-Flow capture kit (Magnetic streptavidin beads, CD63-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW400A-1 | CD81 Exo-Flow capture kit (Magnetic streptavidin beads, CD81-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW500A-1 | Rab5b Exo-Flow capture kit (Magnetic streptavidin beads, Rab5b-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW600A-1 | HLA-G Exo-Flow capture kit (Magnetic streptavidin beads, HLA-G-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW610A-1 | CD14 Exo-Flow capture kit (Magnetic streptavidin beads, CD14-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW620A-1 | CD68 Exo-Flow capture kit (Magnetic streptavidin beads, CD68-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW630A-1 | EpCAMExo-Flow capture kit (Magnetic streptavidin beads, EpCAM-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
EXOFLOW650A-1 | Alpha-2-Macroglobulin (A2M) Exo-Flow capture kit (Magnetic streptavidin beads, A2M-biotin capture antibody, Wash an Elution Buffers, Exo-FITC stain) | 10 reactions | 6576.00 |
CSFLOWBASICA-1 | Basic Exo-Flow kit without any coupled antibodies (Uncoupled magnetic streptavidin beads, Wash and Elution Buffers, Exo-FITC stain) | 100 reactions | 11313.00 |
货号 | 名称 | 规格 | 价格 |
EXOFLOW700A-1 | Exo-Flow Multifunctional Magnetic Stand | 1 Kit | 4525.00 |
EXOFLOW800A-1 | Exo-FITC Exosome FACS stain | 20 reactions | 2474.00 |
EXOFLOW810A-1 | Exo-APC Exosome FACS stain | 20 reactions | 2474.00 |
Exo-Flow 96/32 IP试剂盒可从血浆样本、使用ExoQuick-TC或超速离心富集的细胞培养上清(尿液、脑脊髓液等)Exosomes产物样本中高通量、直接免疫沉淀Exosomes。试剂盒中的磁珠已经预结合了CD9、CD63或者CD81用于选择性捕获特定的Exosomes亚型,直接将上述样本添加入磁珠中即可免疫沉淀Exosomes。96孔板或32孔板形式产品可用于同时处理多个样本。得到Exosomes具有完整形态,可用于下游Exosomes RNA和蛋白分析。
产品信息
货号 | 名称 | 规格 | 价格 |
EXOFLOW32A-CD9 | Exo-Flow 32 CD9 IP kit | 32 rxns | 7699.00 |
EXOFLOW32A-CD63 | Exo-Flow 32 CD63 IP kit | 32 rxns | 7699.00 |
EXOFLOW32A-CD81 | Exo-Flow 32 CD81 IP kit | 32 rxns | 7699.00 |
EXOFLOW96A-CD9 | Exo-Flow 96 CD9 IP kit | 96 rxns | 20429.00 |
EXOFLOW96A-CD63 | Exo-Flow 96 CD63 IP kit | 96 rxns | 20429.00 |
EXOFLOW96A-CD81 | Exo-Flow 96 CD81 IP kit | 96 rxns | 20429.00 |
货号 | 名称 | 规格 | 价格 |
EXOFLOWMAG-1 | ExoFlowMag96 96-well plate magnet | 1 Kit | 8350.00 |
步骤二:Exosome Antibodies and ELISA Kits – 分析、定量 Exosome Protein Markers
(一)ELISA定量分析
SBI公司研发的ExoELISA试剂盒适用于所有类型细胞分泌的任何方法分离得到的Exosome,并且有三种Exosome表面特殊蛋白CD63,CD9,CD81供选择。
产品信息
货号 | 产品名称 | 规格 | 价格(元) |
EXOCET96A-1 | EXOCET Exosome Quantitation Kit | 96 reactions | 6123.00 |
FCET96A-1 | FluoroCet Exosome Quantitation kit | 96 reactions | 8955.00 |
EXOEL-CD63A-1 | Exosome ELISA Complete Kit (CD63 detection) | 96 reactions | 9604.00 |
EXOEL-CD81A-1 | Exosome ELISA Complete Kit (CD81 detection) | 96 reactions | 9604.00 |
EXOEL-CD9A-1 | Exosome ELISA Complete Kit (CD9 detection) | 96 reactions | 9604.00 |
关联产品
(二)CETP活性定量分析
SBI公司已通过研究证明,对于血清、干细胞、肿瘤细胞系、Msc来源的Exosome中富含AchE(乙酰辅酶A乙酰胆碱酯)通过检测AchE的活性可直接对Exosome进行定量。
产品信息
货号 | 名称 | 规格 | 价格(元) |
EXOCET96A-1 | EXOCET Exosome Quantitation Kit | 96 rxns | 6123.00 |
步骤三:Exosome CytoTracers – 照亮细胞里的 Exosomes,让它无所遁形
步骤四:纯化、放大 Exosome RNAs (exoRNAs) 产量,SeraMir Exosome RNA Purification, Amplification Kit
步骤五:Exosome miRNA qPCR Profiler Assay Set – 一次检測 380 种 miRNA 表现量